Functional Expression of the Alkane-Inducible Monooxygenase System of the Yeast: Candida tropicalis IN Saccharomyces cerevisiae |
| |
Authors: | D. Sanglard I. Beretta M. Wagner O. K ppeli A. Fiechter |
| |
Affiliation: | D. Sanglard ,I. Beretta,M. Wagner,O. Kä,ppeli,A. Fiechter |
| |
Abstract: | The genes for the alkane-inducible monooxygenase system of the yeast Candida tropicalis, namely a cytochrome P450alk (P450alk) and a NADPH cytochrome P450 oxidoreductase (NCPR) gene, were transferred in Saccharomyces cerevisiae. The P450alk gene was expressed in this host with the help of the yeast alcohol dehydrogenase I (ADHI) promoter and terminator, whereas the NCPR gene could be expressed with its own structural elements. The presence of P450alk in S. cerevisiae microsomal fractions resulted in a new acquired lauric acid terminal hydroxylation activity. Moreover, the same activity, coupled with the appearance of 12-hydroxylauric acid derivatives, could be obtained by the addition of lauric acid to intact cells expressing P450alk. The coordinate expression of the P450alk and NCPR genes in S. cerevisiae elevated the turnover rate of the P450alk monooxygenase activity about 2-fold. |
| |
Keywords: | Candida tropicalis cytochrome P450 lauric acid hydroxylation oxidoreductase recombinant DNA Saccharomyces cerevisiae |
本文献已被 InformaWorld 等数据库收录! |
|