A comparative study of protein synthesis in in vitro systems: from the prokaryotic reconstituted to the eukaryotic extract-based |
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Authors: | Jason R Hillebrecht Shaorong Chong |
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Affiliation: | (1) New England Biolabs, 240 County Road, Ipswich, MA 01938, USA;(2) Eedetics, 1200 Soldiers Field Road, Boston, MA 02134, USA |
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Abstract: | Background Cell-free protein synthesis is not only a rapid and high throughput technology to obtain proteins from their genes, but also provides an in vitro platform to study protein translation and folding. A detailed comparison of in vitro protein synthesis in different cell-free systems may provide insights to their biological differences and guidelines for their applications. |
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