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Isolation and cloning of Streptomyces terminal fragments |
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| Authors: | Alexander Stoll Lada-Ivana Horvat Sonia AR Lopes-Shikida Gabriel Padilla John Cullum |
| Institution: | (1) LB Genetik, University of Kaiserslautern, Postfach 3049, D-67653 Kaiserslautern, Germany;(2) Chemistry Division, Department of Biotechnology, Institute of Technological Research of the São Paulo State -IPT-, Brazil;(3) Department of Microbiology, Institute of Biomedical Science, University of São Paulo, Brazil;(4) LB Genetik, University of Kaiserslautern, Postfach 3049, D-67653 Kaiserslautern, Germany |
| Abstract: | Streptomyces species have a linear chromosome of approximately 8 Mb in size. Many strains also carry linear plasmids. Most of these linear elements contain terminal proteins covalently bound to the 5 ends of the DNA. Using a method for the visualisation of terminal DNA fragments in agarose gels, it was possible to see three fragments in S. rimosus and five fragments in S. avermitilis. The method was also used to clone the 298 bp BamHI fragment carrying the left end of plasmid SLP2. Analysis of the sequence showed that the end resembled other Streptomyces chromosome and plasmid ends, but there were eight palindromes (instead of seven) and a tandem duplication of a 14 bp sequence. |
| Keywords: | SLP2 Streptomyces terminal fragments |
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