Genetic transformation in the grain legume Cicer arietinum L. (chickpea) |
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Authors: | Giovanna S Fontana Luigi Santini Sofia Caretto Giovanna Frugis Domenico Mariotti |
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Institution: | (1) Istituto di Radiobiochimica ed Ecofisiologia vegetali, CNR, via Salaria Km 29.300, 00016 Monterotondo scalo (RM), Italy |
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Abstract: | In the grain legume Cicer arietinum L. (chickpea), the seed-derived embryo axes deprived of the apical meristem were able to regenerate adventitious shoots on Murashige and Skoog (1962) medium supplemented with kinetin. This protocol was suitable for Agrobacterium-mediated gene transfer by the co-cultivation technique. Chickpea transgenic plants showed neomycin phosphotransferase II and ß-glucuronidase activities and the presence in their genome of integrated bacterial DNA.Abbreviations 6-BAP
6-benzyl-aminopurine
- CaMV
cauliflower mosaic virus
- GUS
ß-glucuronidase
- IAA
indole-3-acetic acid
- Kn
kanamycin
- MU
methyl umbelliferone
- NAA
naphthaleneacetic acid
- NPTII
neomycin phosphotransferase II |
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Keywords: | |
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