Characterization of the vesicular monoamine transporter in cultured rat sympathetic neurons: persistence upon induction of cholinergic phenotypic traits |
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| Authors: | D Scherman M J Weber |
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| Institution: | 1. Institut de Biologie Physico-Chimique, 13, rue Pierre et Marie Curie, 75005 Paris, France;2. Laboratoire de Pharmacologie et de Toxicologie fondamentales, 205, route de Narbonne, 31400 Toulouse, France;1. Center for Artificial Intelligence in Drug Discovery, School of Medicine, Case Western Reserve University, Cleveland, OH, USA;2. Center for Addiction Research, University of Cincinnati College of Medicine, Cincinnati, OH, USA;3. Cleveland Clinic Lerner College of Medicine, Case Western Reserve University, Cleveland, OH, USA;4. Center for the Clinical Trials Network (CCTN), National Institute on Drug Abuse (NIDA), National Institutes of Health (NIH), Bethesda, MD, USA;5. Department of Psychiatry, New York State Psychiatric Institute, Irving Medical Center, Columbia University, New York, NY, USA;6. Department of Psychiatry and Behavioral Science, School of Medicine, University of Washington, Seattle, WA, USA;7. Addiction Medicine Section, Department of Medicine, Oregon Health & Science University, Portland, OR, USA;8. Department of Psychiatry and Behavioral Sciences, College of Medicine, Medical University of South Carolina, Charleston, SC, USA;9. Columbia University Division on Substance Use Disorders, and Research Scientist, New York State Psychiatric Institute, New York, NY, USA;10. Center for Community Health Integration, School of Medicine, Case Western Reserve University, Cleveland, OH, USA;11. Center for Clinical Informatics Research and Education, The Metro Health System, Cleveland, OH, USA;1. Center for Laboratory Animal Science, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama 359-8513, Japan;2. Department of Anatomy (Cell Biology), Iwate Medical University, 2-1-1 Nishitokuta, Yahaba, Iwate 028-3694, Japan;3. Laboratory for Anatomy and Physiology, Department of Sport and Medical Science, Kokushikan University, 7-3-1 Nagayama, Tama, Tokyo 206-8515, Japan;4. Laboratory of Veterinary Anatomy and Cell Biology, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka, Iwate 020-8550, Japan |
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| Abstract: | The binding of 3H]dihydrotetrabenazine (3H]TBZOH), a specific ligand of the reserpine-sensitive monoamine transporter in brain and adrenal medulla storage vesicles, has been measured in cultured sympathetic neurons from newborn rat in relation to their neurotransmitter phenotype. As shown previously, neurons cultured in the absence of muscle-conditioned medium displayed high activities in catecholamine synthesizing enzymes and low levels of choline acetyltransferase, and neurons cultured in conditioned medium displayed the reverse pattern (J. P. Swerts, A. Le Van Thai, A. Vigny, and M. J. Weber, Dev. Biol. 100, 1-11, 1983). The density of 3H]TBZOH binding sites as well as their subcellular distribution were identical in both types of cultures. Two other structures rich in choline acetyltransferase, the electric organ of Torpedo and the ciliary ganglion of the chick embryo did not contain measurable amounts of 3H]TBZOH binding sites, suggesting that the monoamine transporter is not an ubiquitous component of cholinergic synaptic vesicles. These data suggest that the synthesis of the monoamine transporter in sympathetic neurons is not coregulated with the syntheses of the three norpinephrine synthesizing enzymes. It is proposed that the same population of synaptic vesicles can accumulate acetylcholine or catecholamine, depending only upon which neurotransmitter synthesizing enzymes are expressed by sympathetic neurons. |
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