Chemical modification of photosystem II core complex pigments with sodium borohydride

The reaction of the irreversible chemical reduction of the 13¹-keto C=O group of pheophytin a (Pheo a) with sodium borohydride in reaction centers (RCs) of functionally active spinach photosystem II (PS II) core complexes was studied. Stable, chromatographically purified PS II core complex preparations with altered chromophore composition are obtained in which ～25% of Pheo a molecules are modified to 13¹-deoxo-13¹-hydroxy-Pheo a. Some of the chlorophyll a molecules in the complexes were also irreversibly reduced with borohydride to 13¹-deoxo-13¹-hydroxy-chlorophyll a. Based on the results of comparative study of spectral, biochemical, and photochemical properties of NaBH₄-treated and control preparations, it was concluded that: (i) the borohydride treatment did not result in significant dissociation of the PS II core complex protein ensemble; (ii) the modified complexes retained the ability to photoaccumulate the radical anion of the pheophytin electron acceptor in the presence of exogenous electron donor; (iii) only the photochemically inactive pheo-phytin Pheo_D2 is subjected to the borohydride treatment; (iv) the Q_x optical transition of the Pheo_D2 molecule in the RC of PS II core complexes is located at 543 nm; (v) in the Q_y spectral region, Pheo_D2 probably absorbs at ～680 nm.