| 一株新的重组人源过氧化氢酶基因工程菌的构建和遗传稳定性研究 |
| |
| 引用本文： | 史训龙,施志慧,周伟,叶丽,朱海燕,冯美卿,周珮. 一株新的重组人源过氧化氢酶基因工程菌的构建和遗传稳定性研究[J]. 工业微生物, 2011, 41(3): 1-6. DOI: 10.3969/j.issn.1001-6678.2011.03.001 |
| |
| 作者姓名： | 史训龙 施志慧 周伟 叶丽 朱海燕 冯美卿 周珮 |
| |
| 作者单位： | 复旦大学,药学院,生物合成教研室,上海,200032 |
| |
| 摘 要： | 利用pPICZαA作为新的表达载体和表达宿主酵母GS115,成功地构建了新的人源过氧化氢酶表达工程菌G13,并考察了新的工程菌G13的遗传稳定性.通过对新的重组菌整合质粒的酶切,PCR鉴定,及SDS-PAGE电泳、单抗dot-blot证实了该重组菌质粒构建正确,可以有效表达重组的人源过氧化氢酶.新的重组酵母菌G13在连...
|
| 关 键 词： | 人源过氧化氢酶 毕氏酵母 基因工程菌 重组质粒 遗传稳定性 |
Studies on construction of human catalase-producing recombinant yeast strain G13 and its genetic stability |
| |
| SHI Xun-long,SHI Zhi-hui,ZHOU Wei,YE Li,ZHU Hai-yan,FENG Mei-qing,ZHOU Pei. Studies on construction of human catalase-producing recombinant yeast strain G13 and its genetic stability[J]. Industrial Microbiology, 2011, 41(3): 1-6. DOI: 10.3969/j.issn.1001-6678.2011.03.001 |
| |
| Authors: | SHI Xun-long SHI Zhi-hui ZHOU Wei YE Li ZHU Hai-yan FENG Mei-qing ZHOU Pei |
| |
| Affiliation: | Wei YE Li ZHU Hai-yan FENG Mei-qing ZHOU Pei (Department of Drug Biosynthesis, School of Pharmacy, Fudan University, 826 Zhang Heng Road, Shanghai 200032, China) |
| |
| Abstract: | Using pPICZaA as the new yeast expressing vector, the genetic engineering strain GSll5-pPICZaA CAT producing human catalase was successfully constructed, and the genetic stability of the strain G13 was studied. Restriction endonuelease digestion and PCR analysis showed that the recombinant plasmid was properly constructed, and the SDS-PAGE convinced that the strain could express the target protein. The recombinant strain G13 was continuously incubated for 24 generations. The results showed the plasmid had structural stability and the recombinant strain could maintain human catalase activity. |
| |
| Keywords: | human catalase Pichia genetic engineering strain recombinant plasmid genetic stability |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
