Synthesis of linked triple helical DNAs possessing high affinity to triple helical DNA binding protein |
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| Authors: | Shibata Aya Ueno Yoshihito Shinbo Koichiro Nakanishi Masayuki Matsuda Akira Kitade Yukio |
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| Affiliation: | Department of Biomolecular Science, Faculty of Engineering, Gifu University, Yanagido, Gifu 501-1193, Japan. |
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| Abstract: | The synthesis of triplexes possessing an anthraquinonyl group and composed of branched oligonucleotides (ONs) is described. Binding ability of a triplex-binding protein (MBP-LOR3(ARF)) to the triplexes was evaluated by an electrophoretic mobility shift assay (EMSA). It was found that the triplex, which has an anthraquinonecarbonyl group at the 5'-end of the third strand and is connected with the pentaerythritol linker, has greater affinity to the protein than an unmodified triplex. |
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