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Intraspecific protoplast fusion of amylase-producing strains of Candida fennica
Authors:V R Linardi  C M M C Carvalho  A A M Dias
Institution:(1) Departamento de Microbiologia, Universidade Federal de Minas Gerais, Cx.P.2486, 30161-970 Belo Horizonte, Brazil
Abstract:The production of agr-amylase was increased by protoplast fusion of auxotrophic mutants of Candida fennica FTPT-8903. One prototrophic fusant was 90% and 32% more efficient in producing agr-amylase in semi-solid and liquid fermentation, respectively, than the parental strains. Protoplast fusion did not significantly stimulate the synthesis of glucoamylase in the fusants.
Keywords:Amylase  Candida fennica  liquid fermentation  protoplast fusion  semi-solid fermentation
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