Metabolic Consequences of Altered PhosphoenolpyruvateCarboxykinase Activity in Corynebacterium glutamicum Reveal Anaplerotic Regulation Mechanisms in Vivo |
| |
Authors: | S ren Petersen, Christina Mack, Albert A. De Graaf, Christian Riedel, Bernhard J. Eikmanns,Hermann Sahm |
| |
Affiliation: | Institute of Biotechnology 1, Research Center Jülich, 52425 Jülich, Germany. |
| |
Abstract: | Corynebacterium glutamicum possesses high in vivo activity of the gluconeogenic phosphoenolpyruvate carboxykinase (PEPCk) during growth on glucose, resulting together with anaplerotic carboxylation reactions in a PEP/pyruvate/oxaloacetate substrate cycle. The present study investigated the changes in intracellular fluxes and metabolite concentrations that are caused by altered PEPCk activity in L-lysine-producing C. glutamicum MH20-22B, applying a recently developed (13)C labeling-based strategy for anaplerotic flux resolution and quantification. Abolition of PEPCk activity by deletion of the respective pck gene resulted in increased intracellular concentrations of oxaloacetate L-aspartate, alpha-ketoglutarate, pyruvate, and L-lysine and in a 60% enhanced flux toward L-lysine biosynthesis, whereas increasing the PEPCk activity by pck overexpression had opposite effects. The results of the combined measurements of enzyme activities, in vivo fluxes, and metabolite concentrations were exploited to elucidate the in vivo regulation of anaplerotic reactions in C. glutamicum, and implications for the metabolic engineering of amino-acid-producing strains are discussed. |
| |
Keywords: | Corynebacterium glutamicum metabolic flux analysis intracellular metabolite concentrations anaplerotic reactions phosphoenolpyruvate carboxykinase substrate cycling in vivo regulation |
本文献已被 ScienceDirect 等数据库收录! |
|