| 禽类原始生殖细胞的迁移能力 |
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| 引用本文： | 李赞东,李相芝,赵东风,Takashi KUWANA.禽类原始生殖细胞的迁移能力[J].动物学报,2003,49(6):868-872. |
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| 作者姓名： | 李赞东 李相芝 赵东风 Takashi KUWANA |
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| 作者单位： | 1. 中国农业大学生物学院,北京,100094
2. National Institute for Minamata Disease, Kumamoto,867-0008, Japan |
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| 基金项目： | 国家自然科学基金 (No .3 9980 0 3 0 )资助项目，国家“863”计划(No .2 0 0 1AA2 13 13 1)资助项目~~ |
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| 摘 要： | Blood samples were collected from chicken embryos at stage 11-15,and labeled with fluorescent dye PKH26.Primordial germ cells (PGCs)were then isolated from blood samples by nycodenz density gradient centrifugation.After PGCs were labeled and isolated,about 200 PGCs in one microliter were injected into the subgerminal cavity of quail blastoderm at stageX.After 48 hours incubation,chicken PGCs were identified by fluorescent microscopy.Red fluorescence emitted from PKH26 labeled chicken PGCs was observed in the head, the heart and the developing gonadal anlage of quail embryos.The result suggests that chicken PGCs still keep migration ability after 56 hoursActa Zoologica Sinica 49(6):868-872,2003].
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| 关 键 词： | 鸡 鹌鹑 原始生殖细胞 生殖原基 荧光色素 禽类 |
Migration of avian primordial germ cells |
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| Abstract: | |
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| Keywords: | Chicken Quail Primordial germ cells(PGCs) Gonadal anlage Fluorescent dye |
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