Hypercholesterolemia and LDL receptor mRNA expression: modulation by selenium supplementation |
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Authors: | Sanjiv Dhingra Mohinder P Bansal |
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Institution: | (1) Department of Biophysics, Punjab University, Chandigarh, 160014, India |
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Abstract: | Selenium (Se) status has been associated with cardiovascular disorders. Present study was aimed to elucidate the protective
role of Se supplementation on LDL receptor (LDL-R) activity as well as mRNA expression during experimental hypercholesterolemia
in SD male rats. Animals were fed 0.2 and 1 ppm Se supplemented control diet as well as 2% cholesterol supplemented diet for
3 months. LDL-R activity was measured in-vivo by injecting radiolabeled LDL to rats and decrease in counts per minute with time was taken as a measure of LDL clearance
and in turn LDL-R activity. LDL-R mRNA expression was studied by RT-PCR. LDL-R activity and mRNA expression decreased significantly
on 2% cholesterol supplemented diet feeding. On 1 ppm Se supplementation LDL-R activity as well as mRNA expression increased
significantly. Present results demonstrate that Se supplementation upto 1 ppm is responsible for up regulation of LDL-R activity
as well as mRNA expression, during hypercholesterolemia. These findings highlight the therapeutic potential of Se supplementation
in lipid metabolism. |
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Keywords: | cholesterol hypercholesterolemia LDL-R selenium |
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