| 基于GFP的FRET应用 |
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| 引用本文： | 周炜,曾绍群,张智红,陈同生,骆清铭. 基于GFP的FRET应用[J]. 生命科学, 2002, 14(1): 56-58 |
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| 作者姓名： | 周炜 曾绍群 张智红 陈同生 骆清铭 |
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| 作者单位： | 华中理工大学生命科学与技术学院生物医学光子学教育部重点实验室,武汉,430074 |
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| 基金项目： | 国家自然科学基金项目（39870205），国家杰出青年基金（60025514），高等学校博士学科点专项科研基金（980487 |
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| 摘 要： | 绿色荧光蛋白（GFP）是一种活性荧光标记，已被用来研究基因表达、分子定位，蛋白质折叠和转运；荧光共振能量转移（FRET）是一种无损伤的光学检测方法，能检测到小于纳米的距离变化。将GFP的活性定位标记功能与FRET的高分辨率相结合。为活体研究生物分子的功能和命运开创了新的篇章。作者在介绍GFP和FRET原理的基础上，综述了基于GFP的FRET在蛋白酶活性，蛋白质间相互作用 构象改变研究中的应用。
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| 关 键 词： | 绿色荧光蛋白 荧光共振能量转移 构象 蛋白质相互作用 GFP FRET 应用 |
| 文章编号： | 1004-0374(2002)01-0056-03 |
| 修稿时间： | 2000-07-03 |
Applications of FRET based on GFP |
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| ZHOU Wei,ZENG Shao-Qun,ZHANG Zhi-Hong,CHEN Tong-Sheng,LUO Qing-Ming. Applications of FRET based on GFP[J]. Chinese Bulletin of Life Sciences, 2002, 14(1): 56-58 |
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| Authors: | ZHOU Wei ZENG Shao-Qun ZHANG Zhi-Hong CHEN Tong-Sheng LUO Qing-Ming |
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| Abstract: | As a genetically encoded tag, green fluorescent protein(GFP) has served as a versatile tool in cell biology for studying gene expression, molecular locating, protein folding and trafficking. Fluorescence resonance energy transfer(FRET) is a non-invasive optical method which can detect distance change as short as one nanometer. The combination of GFP technology and FRET microscope offered high(subcellular) resolution and sensitivity in living cells, and thereby creates new horizons for studying the fate and function of biomolecules in vivo. This review introduced GFP and the theory of FRET. Applications of GFP-based FRET in the study of protein-protein interactions, protein conformational changes and proteolytic processing was also summarized. |
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| Keywords: | GFP FRET conformation protein interaction |
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