The PSO3 gene is involved in error-prone intragenic recombinational DNA repair in Saccharomyces cerevisiae |
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Authors: | Heloisa Helena Rodrigues de Andrade, Ethel Moustacchi Joao Antonio Pê gas Henriques |
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Affiliation: | (1) Instituto de Biociências, Departamento de Genetica, Universidade Federal do Rio Grande do Sul (UFRGS), Rua Prof. Annes Dias, 112/15 andar, 90001 Porto Alegre RS, Brazil;(2) Institut Curie, Section de Biologic, UA 1292 CNRS, 26 rue d'Ulm, F-75231 Paris Cedex 05, France;(3) Instituto de Biociências, Departamento de Fisiologia, Farmacologia e Biofisica, Universidade Federal do Rio Grande do Sul (UFRGS), Rua Prof. Sarmento Leite, 500, 90049 Porto Alegre RS, Brazil |
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Abstract: | Summary The induction of gene conversion and mitotic crossing-over by photoaddition of psoralens, 254 nm ultraviolet radiation, and nitrogen mustards was determined in diploid cells homozygous for the pso3-1 mutation and in the corresponding wild type of Saccharomyces cerevisiae. For these different agents, the frequency of non-reciprocal events (conversion) is reduced in the pso3-1 mutant compared to the wild type. In contrast, the frequency of reciprocal events (crossing-over) is increased at a range of doses. These observations, together with the block in induced mutagenesis for both reverse and forward mutations previously reported for the pso3-1 mutant, suggest that the PS03 gene product plays a role in mismatch repair of short patch regions. The block in gene conversion in the pso3 homozygous diploid leads, in the case of nitrogen mustards, to specific repair intermediates which are lethal to the cells. |
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Keywords: | Gene conversion Crossing-over Mismatch repair Saccharomyces cerevisiae Psoralens |
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