Effects of various agents on the R bodies of certain bacteria |
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| Affiliation: | 1. Department of Internal Medicine III, Cardiology, Angiology & Pulmonology, Heidelberg University Hospital, Heidelberg, Germany;2. Department of Medical Sciences, Uppsala University, Uppsala, Sweden;3. Uppsala Clinical Research Center, Uppsala University, Uppsala, Sweden;4. Department of Medicine, University of Maryland School of Medicine, Baltimore, MD, United States;5. Department of Emergency and Critical Care Medicine, Community Hospital, Paracelsus Medical University, Nuremberg, Germany;6. Semicritical Unit, Hospital de Sant Pau &, Institut d''Investigacions Biomèdiques Sant Pau, Barcelona, Spain;7. Department of Biomedical and Clinical Sciences ‘Luigi Sacco’, University of Milan Medical School, Milano, Italy;8. Department of Laboratory Medicine, University Hospital of Padova, Padua, Italy;9. Department of Acute Medicine, Cliniques Universitaires St-Luc, Université Catholique de Louvain, Brussels, Belgium;10. Department of Medicine, KarolinskaInstitutet, Huddinge, Sweden;11. Liverpool Hospital, University of New South Wales, Sydney, Australia;12. Department of Pathology, University of Maryland School of Medicine, Baltimore, MD, United States;13. Central Manchester University Hospitals NHS Foundation Trust, United Kingdom;14. Henry Ford Heart and Vascular Institute, Henry Ford Health System, Detroit, MI, United States;15. Roche Diagnostics Germany, Penzberg, Germany;p. Cardiology & Cardiovascular Research Institute Basel, University Hospital Basel, Switzerland |
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| Abstract: | We have shown that urea and temperature affect the unrolling of the R bodies from the bacteria Caedibacter taeniospiralis, Pseudomonas taeniospiralis and Pseudomonas avenae. The R bodies of P. taeniospiralis are not, however, affected by pH changes nor by EDTA or EGTA unlike the other two types of R body. Antibodies prepared against the R bodies of C. taeniospiralis have been shown, following gold-labelling and electron microscopy, to be specific for the proteins of the homologous R body. Pre-treatment of R bodies with pronase or sodium periodate had no effect on these reactions. |
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