Induction of high polyploidy in Phaseolus cell cultures by the protein kinase inhibitor,K-252a |
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Authors: | Walter Nagl |
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Institution: | (1) Department of Biology, University of Kaiserslautern, P.O. Box 3049, W-6750 Kaiserslautern, FRG |
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Abstract: | Summary Somatic polyploidy of species-specific and tissue-specific degrees occurs in almost all plant species studied so far, but nearly nothing is known about the control mechanisms switching the mitotic cycle to an endoreduplication cycle. In order to search for a possible role of the cdc2 kinase, cell suspension cultures of the Runner bean, Phaseolus coccineus (Leguminosae) were treated with K-252a, an inhibitor of protein kinase activity. The treatment resulted in continuous cell cycles without mitosis, and hence induced polyploidy levels up to 2048C. It is, therefore, suggested that phosphorylation of a protein kinase, probably of the cell cycle-important p34cdc2 type, is involved in the control of endoreduplication. |
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Keywords: | Cdc2 Cell cycle switch Endoreduplication Plants Staurosporine analogue Suspension culture |
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