Following the rule: formation of the 6-helix bundle of the fusion core from severe acute respiratory syndrome coronavirus spike protein and identification of potent peptide inhibitors |
| |
Authors: | Zhu Jieqing Xiao Gengfu Xu Yanhui Yuan Fang Zheng Congyi Liu Yueyong Yan Huimin Cole David K Bell John I Rao Zihe Tien Po Gao George F |
| |
Institution: | Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China. |
| |
Abstract: | Severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) is a newly identified member of Family Coronaviridae. Coronavirus envelope spike protein S is a class I viral fusion protein which is characterized by the existence of two heptad repeat regions (HR1 and HR2) (forming a complex called fusion core). Here we report that by using in vitro bio-engineering techniques, SARS-CoV HR1 and HR2 bind to each other and form a typical 6-helix bundle. The HR2, either as a synthetic peptide or as a GST-fusion polypeptide, is a potent inhibitor of virus entry. The results do show that SARS-CoV follows the general fusion mechanism of class I viruses and this lays the ground for identification of virus fusion/entry inhibitors for this devastating emerging virus. |
| |
Keywords: | SARS-CoV Coronavirus Spike(S) protein Fusion core Heptad repeat (HR1 and HR2) Inhibition |
本文献已被 ScienceDirect PubMed 等数据库收录! |