Plant regeneration from embryogenic cell suspension cultures of <Emphasis Type="Italic">Lolium temulentum</Emphasis> |
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Authors: | Email author" target="_blank">Z?Y?WangEmail author M?Scott A?Hopkins |
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Institution: | (1) Forage Biotechnology Group, The Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, 73401 Ardmore, OK |
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Abstract: | Summary
Lolium temulentum L. (Darnel ryegrass) is a self-fertile and diploid grass species with a relatively short life cycle. We propose to use L. temulentum as a model system for genetic manipulation studies in forage and turf grasses, since most of the important grasses are outcrossing,
require vernalization to flower, and in some cases are polyploid. As the first step to develop an efficient regeneration and
transformation system, we performed a large-scale genotype screening for tissue culture responses using 46 L. temulentum accessions. Embryogenic callus formation frequency ranged from <1% to 11% across all accessions tested. Embryogenic calluses
of a few responsive accessions were used to establish cell suspension cultures. The regeneration frequency of green plantlets
from the established cell suspension ranged from 15% to 39%. After transferring the regenerants to the greenhouse, fertile
plants were readily obtained without any vernalization treatment. This efficient plant regeneration system is being used for
genetic transformation studies. With the development of genomics approaches for the improvement of forage and turf grasses,
L. temulentum could serve as a model system for testing gene functions. |
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Keywords: | L temulentum forage and turf grasses plant regeneration callus cell suspension |
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