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Molecular imaging of amyloid beta peptides in mouse brain sections using mass spectrometry
Authors:Stoeckli Markus  Staab Dieter  Staufenbiel Matthias  Wiederhold Karl-Heinz  Signor Luca
Affiliation:Department of Chemistry, East China Normal University, Shanghai, People's Republic of China.
Abstract:In this paper, a reverse-phase high-performance liquid chromatographic method using a chemically modified electrode coupled with microdialysis was developed to study the effect of electromagnetic impulse (EMI) on monoamine neurotransmitter metabolism in nerve cells. To detect the monoamines and their metabolites, a poly (para-aminobenzoic acid) (P-pABA)-modified electrode was prepared. The modified electrode exhibited efficiently electrocatalytic oxidation for monoamines and their metabolites with relatively high sensitivity, stability, and long life. Nerve cells were primarily cultured. EMI was radiated to three experimental model nerve cells: (i) on mature nerve cells, (ii) on the culture medium, and (iii) on juvenile nerve cells for various periods of time. Then the levels of monoamines in the culture medium were detected by high-performance liquid chromatography-electrochemical detection. The data indicated that electromagnetic fields could influence neurotransmitter metabolism by direct effect on nerve cells or effect on the nutrient medium and that the effect was not only relevant with the length of radiation time, but also with the growing state of the nerve cells.
Keywords:P-pABA-modified electrode (P-pABA ME)   HPLC-ECD   Microdialysis   Monoamines   Electromagnetic impulse   Nerve cells
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