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Synergism between hydrogen sulfide (H(2)S) and nitric oxide (NO) in vasorelaxation induced by stonustoxin (SNTX), a lethal and hypotensive protein factor isolated from stonefish Synanceja horrida venom
Authors:Liew H C  Khoo H E  Moore P K  Bhatia M  Lu J  Moochhala S M
Institution:Department of Biochemistry, National University of Singapore, Blk MD4A, 10 Kent Ridge Crescent, Singapore.
Abstract:Stonustoxin (SNTX) is a 148 kDa, dimeric, hypotensive and lethal protein factor isolated from the venom of the stonefish Synanceja horrida. SNTX (10-320 ng/ml) progressively causes relaxation of endothelium-intact, phenylephrine (PE)-precontracted rat thoracic aortic rings. The SNTX-induced vasorelaxation was inhibited by L-N(G)-nitro arginine methyl ester (L-NAME), suggesting that nitric oxide (NO) contributes to the SNTX-induced response. Interestingly, D, L-proparglyglycine (PAG) and beta-cyano-L-alanine (BCA), irreversible and competitive inhibitors of cystathionine-gamma-lyase (CSE) respectively, also inhibited SNTX-induced vasorelaxation, indicating that H(2)S may also play a part in the effect of SNTX. The combined use of L-NAME with PAG or BCA showed that H(2)S and NO act synergistically in effecting SNTX-induced vasorelaxation.
Keywords:l-NAME" target="_blank">l-NAME  l-NG-nitro arginine methyl ester" target="_blank">l-NG-nitro arginine methyl ester  PAG  d" target="_blank">d  l-proparglyglycine" target="_blank">l-proparglyglycine  BCA  l-alanine" target="_blank">β-cyano-l-alanine  PE  l-phenylephrine" target="_blank">l-phenylephrine  CSE  Cystathionine-γ-lyase  NOS  Nitric Oxide Synthase  ACh  Acetylcholine  
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