Synergism between hydrogen sulfide (H(2)S) and nitric oxide (NO) in vasorelaxation induced by stonustoxin (SNTX), a lethal and hypotensive protein factor isolated from stonefish Synanceja horrida venom |
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Authors: | Liew H C Khoo H E Moore P K Bhatia M Lu J Moochhala S M |
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Institution: | Department of Biochemistry, National University of Singapore, Blk MD4A, 10 Kent Ridge Crescent, Singapore. |
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Abstract: | Stonustoxin (SNTX) is a 148 kDa, dimeric, hypotensive and lethal protein factor isolated from the venom of the stonefish Synanceja horrida. SNTX (10-320 ng/ml) progressively causes relaxation of endothelium-intact, phenylephrine (PE)-precontracted rat thoracic aortic rings. The SNTX-induced vasorelaxation was inhibited by L-N(G)-nitro arginine methyl ester (L-NAME), suggesting that nitric oxide (NO) contributes to the SNTX-induced response. Interestingly, D, L-proparglyglycine (PAG) and beta-cyano-L-alanine (BCA), irreversible and competitive inhibitors of cystathionine-gamma-lyase (CSE) respectively, also inhibited SNTX-induced vasorelaxation, indicating that H(2)S may also play a part in the effect of SNTX. The combined use of L-NAME with PAG or BCA showed that H(2)S and NO act synergistically in effecting SNTX-induced vasorelaxation. |
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Keywords: | l-NAME" target="_blank">l-NAME l-NG-nitro arginine methyl ester" target="_blank">l-NG-nitro arginine methyl ester PAG d" target="_blank">d l-proparglyglycine" target="_blank">l-proparglyglycine BCA l-alanine" target="_blank">β-cyano-l-alanine PE l-phenylephrine" target="_blank">l-phenylephrine CSE Cystathionine-γ-lyase NOS Nitric Oxide Synthase ACh Acetylcholine |
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