Thermal inactivation and unfolding of a dimeric arginine kinase |
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| Authors: | Qin Guo Xicheng Wang |
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| Institution: | Department of Biological Science and Biotechnology, School of Life Sciences and Engineering, Tsinghua University, Beijing, China. |
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| Abstract: | Thermal inactivation and unfolding of the dimeric arginine kinase (AK) from sea cucumber Stichopus japonicus was investigated. The activation energy was calculated to be 388 kJ/mol. Based on the analysis of the denaturation course at 58 degrees C, a model is suggested for the thermal unfolding of this dimeric AK. In addition, the effect of free Mg(2+) and the potential biological significance on the thermal unfolding of dimeric AK is discussed. |
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