Establishment and Growth of Embryogenic Suspension Cultures of Ocotea catharinensis Mez. (Lauraceae) |
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Authors: | Juliana Righetto Moser Magali Gonçalves Garcia Ana Maria Viana |
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Institution: | (1) Departamento de Botanica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, 88040-900 Florianópolis, SC, Brasil |
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Abstract: | The focus of this study was to test the effects of 2,4-D, sucrose, culture media and initial inocula on the development of
embryogenic suspension cultures of Ocotea catharinensis Mez. (Lauraceae). Suspension cultures were established in half-strength MS medium supplemented with 2% (w/v) sucrose either
in the absence or in the presence of 2.2 μM 2,4-D, when higher cell viability was achieved. Under this culture condition the
maximum fresh weight increase occurred in the fourth week. The cultures were yellow and consisted of a mixture of highly cytoplasmic
single cells and small cell aggregates (<0.25 mm). The best proportion of inoculum per volume of medium for suspension culture
development was 5% (w/w). Suspension cultures consisting of somatic embryos at the globular and cotyledonary stages (structures
ranging from 1 to 3 mm) were successfully established on half-strength MS supplemented with 2% (w/w) sucrose through repetitive
embryogenesis from the desiccated mature somatic embryos used as initial inoculum. The failure to initiate liquid cultures
from non-desiccated mature somatic embryos was overcome by pre-treatment with air desiccation and reduction of the water content
to 6.1 g H2O g−1 dry weight. |
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Keywords: | cell aggregate cell viability desiccation mature somatic embryos |
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