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Automated pneumococcal MLST using liquid-handling robotics and a capillary DNA sequencer |
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| Authors: | Jefferies Johanna Clarke Stuart C Diggle Mathew A Smith Andrew Dowson Chris Mitchell Tim |
| Institution: | (1) Department of Microbiology, Stobhill Hospital, Scottish Meningococcus and Pneumococcus Reference Laboratory, North Glasgow University Hospital NHS Trust, Balornock Rd., G21 3UW Glasgow, UK;(2) Institute of Biomedical and Life Sciences, University of Glasgow, UK;(3) Glasgow Dental School and Hospital, Glasgow;(4) Department of Biological Sciences, Warwick University, UK |
| Abstract: | Multilocus sequence typing (MLST) is used by the Scottish Meningococcus and Pneumococcus Reference Laboratory (SMPRL) as a routine method for the characterization of certain bacterial pathogens. The SMPRL recently started performing MLST on strains of Streptococcus pneumoniae, and here we describe a fully automated method for MLST using a 96-well-format liquid-handling robot and a 96-capillary automated DNA sequencer. |
| Keywords: | Multilocus sequence typing DNA sequencing polymerase chain reaction automation |
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