A Quick-Freeze Method for Making Smear Slides Permanent |
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Authors: | Alan D Conger Lucile M Fairchild |
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Institution: |
a Biology Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee |
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Abstract: | Many types of smear slides can be made permanent rapidly and effectively by substituting for the usual dehydration series a single-step process of freezing the slide on a block of dry ice, placing it immediately in 95% or absolute alcohol, and then mounting it. Advantages of the technic are its speed, the ease of separation of cover slip from slide with a minimum loss of cells, and the superiority of the resulting permanent slides. |
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