Evaluation of adriamycin nephropathy by an in vivo electron paramagnetic resonance |
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| Authors: | Oteki Takaaki Nagase Sohji Yokoyama Hidekatsu Ohya Hiroaki Akatsuka Takao Tada Mika Ueda Atsushi Hirayama Aki Koyama Akio |
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| Affiliation: | Pathophysiology of Renal Diseases, Medical Sciences for Control of Pathological Processes, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Ibaraki, Japan. |
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| Abstract: | A rat model for human minimal change nephropathy was obtained by the intravenous injection of adriamycin (ADR) at 5 mg/kg. By using an in vivo electron paramagnetic resonance (EPR) spectrometer operating at 700 MHz, the temporal changes in signal intensities of a nitroxide radical, 4-hydroxyl-2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPOL), in the kidneys of rats with ADR nephropathy were investigated. The decay rate of the EPR signal intensity obtained in the kidney is indicative of the renal reducing ability. It was found that the reducing ability in the kidney declined on the 7th day after ADR administration and recovered after the 14th day. Impairment of the reducing ability occurred before the appearance of continuous urinary protein. The in vitro EPR study showed that this impairment of in vivo renal reducing ability is related to impairment of the reducing ability in the mitochondria. |
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| Keywords: | Adriamycin nephropathy Electron paramagnetic resonance TEMPOL Mitochondria Reducing ability |
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