Lack of temperature-sensitivity of rat liver pyruvate kinase (Short Communication) |
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| Authors: | Wayne Flory and Roger E Koeppe |
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| Institution: | Department of Biochemistry, Agricultural Experiment Station, Oklahoma State University, Stillwater, Okla. 74074, U.S.A. |
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| Abstract: | Rat liver pyruvate kinase, prepared by Sephadex treatment of a 10(5)g supernatant in phosphate buffer, is quite stable and gives reproducible results when a variety of parameters are altered in the enzyme assay. Incubation of this preparation at 25 degrees C or 0-2 degrees C has no effect on the activation by fructose 1,6-diphosphate or inhibition by ATP or alanine. |
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