Full NMR assignment and revised structure for the capsular polysaccharide from Streptococcus pneumoniae type 15B |
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Authors: | Jones Christopher Lemercinier Xavier |
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Institution: | Laboratory for Molecular Structure, National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Herts EN6 3QG, UK. cjones@nibsc.ac.uk |
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Abstract: | The capsular polysaccharide from Streptococcus pneumoniae Type 15B is a component of the 23-valent polysaccharide vaccine against pneumococcal disease. We report full NMR assignments for the native and de-O-acetylated polysaccharide, and confirm that the phosphorylated substituent is glycerol-2-phosphate rather than phosphocholine, located on O-3 of the side chain beta-Galp residue. The polysaccharide is O-acetylated on the terminal alpha-Gal residue, distributed between O-2, O-3, O-4 and O-6 in a ratio of 6:12:12:55, with approximately 15% of the repeat units not O-acetylated. |
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Keywords: | COSY correlation spectroscopy CPS capsular polysaccharide HSQC heteronuclear single quantum correlation spectroscopy NOE nuclear Overhauser enhancement NOESY NOE spectroscopy ROESY rotating frame NOE spectroscopy TOCSY total correlation spectroscopy |
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