| 内皮素-1对培养新生大鼠心肌细胞原癌基因c-fos表达的诱导 |
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| 引用本文： | Wu B,Wang TH,Zhu XN,Pan JY. 内皮素-1对培养新生大鼠心肌细胞原癌基因c-fos表达的诱导[J]. 生理学报, 1999, 51(1): 19-24 |
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| 作者姓名： | Wu B Wang TH Zhu XN Pan JY |
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| 作者单位： | 中山医科大学生理教研室,广州,510089 |
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| 基金项目： | 国家自然科学基金,中华医学基金,高等学校博士点专项基金 |
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| 摘 要： | 本实验用无血清的培养新生大鼠心肌细胞,探讨内皮素１（ＥＴ１）对原癌基因ｃｆｏｓ表达的作用。结果显示：ＥＴ１可显著诱导ｃｆｏｓ的表达,其表达的高峰在３０ｍｉｎ,２ｈ恢复到正常水平,并呈剂量依赖性反应和被ＥＴＡ的特异性受体拮抗剂ＢＱ１２３所阻断;蛋白激酶Ｃ（ＰＫＣ）激动剂ＰＭＡ可诱导ｃｆｏｓ表达,而ＰＫＣ抑制剂Ｓｔａｕｒｏｓｐｏｒｉｎｅ则可阻断ＥＴ１诱导的ｃｆｏｓ表达;钙通道阻断剂硝苯吡啶预处理心肌细胞对ＥＴ１诱导的心肌细胞的ｃｆｏｓ表达无明显的作用。这些结果提示,ＥＴ１诱导ｃｆｏｓ表达是通过ＥＴＡ受体介导的,ＰＫＣ在此过程中起重要作用。
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| 关 键 词： | 内皮素－1 心肌细胞 c－fos |
ET-1 induces the expression of prooncogene c-fos in cultured neonatal rat myocardial cells |
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| Wu B,Wang T H,Zhu X N,Pan J Y. ET-1 induces the expression of prooncogene c-fos in cultured neonatal rat myocardial cells[J]. Acta Physiologica Sinica, 1999, 51(1): 19-24 |
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| Authors: | Wu B Wang T H Zhu X N Pan J Y |
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| Affiliation: | Department of Physiology, Sun Yat-Sen University of Medical Sciences, Guangzhou 510089. |
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| Abstract: | In the present study, the effect of endothelin 1 (ET 1) on the expression of proto oncogene c fos in cultured neonatal rat myocardial cells was investigated. The results are as follows: ET 1 induced c fos expression in a dose dependent manner. Selective ET A receptor antagonist blocked ET 1 induced responses. Protein kinase C(PKC) agonist PMA induced c fos expression. PKC inhibitor staurosporine blocked ET 1 induced c fos expression. Calcium channel blocker, nifedipine did not significantly affect the expression of c fos induced by ET 1. These results suggest that in cultured neonatal cardiomyocytes, ET 1 induced c fos gene expression is mediated by ET A receptor with the participation of protein kinase C, while the voltage dependent L type Ca 2 channel is not involved. |
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| Keywords: | endothelin 1 c fos cardiomyocyte |
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