| 琯溪蜜柚汁胞在发育与粒化过程中APX活性变化及其同工酶分析 |
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| 引用本文： | 钟凤林,郭志雄,李开拓,王江波,潘东明.琯溪蜜柚汁胞在发育与粒化过程中APX活性变化及其同工酶分析[J].热带亚热带植物学报,2010,18(1):27-31. |
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| 作者姓名： | 钟凤林 郭志雄 李开拓 王江波 潘东明 |
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| 作者单位： | 1. 福建农林大学,园艺学院,福州,350002
2. 福建农林大学,园艺学院,福州,350002;福建农林大学,园艺产品贮藏保鲜研究所,福州,350002 |
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| 基金项目： | 国家科技支撑计划项目 |
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| 摘 要： | 对不同发育时期的琯溪蜜柚(Citrus grandis'Guanximiyou')汁胞进行APX活性测定及同工酶分析.结果表明,随着汁胞的发育,APX活性增大;而柚子衰老腐烂时,APX活性迅速降低.APX同工酶随着汁胞发育而发生变化,花后150 d的APX同工酶增加了1个组分(迁移率0.66),且随着汁胞的成熟,其表达量增加.花后230 d时柚子开始衰老腐烂,花后242 d已难以看到大部分APX同工酶酶谱.粒化汁胞的APX活性比正常汁胞大,同工酶酶谱亮度和清晰度也大,推测琯溪蜜柚汁胞在粒化过程中APX可清除活性氧61由基,抵抗氧化损伤.
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| 关 键 词： | 蜜柚 汁胞 发育 粒化 同工酶 |
| 收稿时间： | 2009/3/15 0:00:00 |
| 修稿时间： | 2009/10/27 0:00:00 |
Activity and Isozyme of Ascorbate Peroxidase in Pomelo (Citrus grandis ' Guanximiyou') during Juice Sac Development and Granulation |
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| ZHONG Feng-lin,GUO Zhi-xiong,LI Kai-tuo,WANG Jiang-bo and PAN Dong-ming.Activity and Isozyme of Ascorbate Peroxidase in Pomelo (Citrus grandis ' Guanximiyou') during Juice Sac Development and Granulation[J].Journal of Tropical and Subtropical Botany,2010,18(1):27-31. |
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| Authors: | ZHONG Feng-lin GUO Zhi-xiong LI Kai-tuo WANG Jiang-bo and PAN Dong-ming |
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| Institution: | ZHONG Feng-lina,GUO Zhi-xionga,LI Kai-tuoa,b,WANG Jiang-boa,PAN Dong-minga,b(a.College of Horticulture,b.Institute of Storage Science , Technology of Horticultural Products,Fujian Agriculture , Forestry University,Fuzhou 350002,China) |
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| Abstract: | The changes in activity and isozyme of ascorbate peroxidase(APX)in pomelo(Citrus grandis 'Guanximiyou')juicy sac were studied at different development stages.The results showed that APX activity increased gradually with juicy sac development and decreased rapidly at aging and rotting stages.The APX isozyme also changed during juicy sac development stages.A new APX isozyme was identified with mobility of 0.66 on 150 days after anthesis,and its expression enhanced with maturity of juicy sac.On 242 days after ... |
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| Keywords: | APX Citrus grandis Juicy sac Development Granulation APX Isozyme |
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