Cloning and Expression of SFRP5 in Tibetan Chicken and its Relationship with IMF Deposition |
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| Authors: | Qian Li Lu-lu Zuo Ya-ou Xu Jiang-jiang Zhu Hong-hai Liao |
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| Affiliation: | 1. College of Life Science and Technology, Southwest University for Nationalities, Chengdu, China;2. Key Laboratory of Sichuan Province for Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Exploitation, Chengdu, China |
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| Abstract: | Secreted frizzled related protein 5 (SFRP5), an anti-inflammatory adipokine, is relevant to the adipocyte differentiation. In order to clarify its role in regulating intramuscular fat (IMF) deposition in Tibetan chicken, the full-length sequence of the Tibetan chicken SFRP5 gene was cloned. The relative expression of SFRP5 gene was detected using quantitative RT-PCR in various tissues of 154 days old Tibetan chicken, as well as in breast muscle, thigh muscle, and adipose tissue at different growth stages. The results showed that SFRP5 gene was expressed in all examined tissues but highly enriched in adipose tissue. Temporal expression profile showed that the expression of SFRP5 was gradually decreased in breast muscle, but was fluctuated in thigh muscle and adipose tissue with the growth of Tibetan chicken. Furthermore, correlation analysis demonstrated that the expression of SFRP5 in breast muscle, thigh muscle and adipose tissue was correlated with IMF content at different levels. The results indicated that Tibetan chicken SFRP5 is involved in IMF deposition. |
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| Keywords: | Adipocyte differentiation expression profile sequence analysis SFRP5 Wnt signaling |
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