Molecular Characterization of Equine APRIL and its Expression Analysis During the Adipogenic Differentiation of Equine Adipose-Derived Stem Cell In Vitro |
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| Authors: | Haitao Wu Xiaolin Bi Fang Cao Cuicui Zhu Hongzhen Liu Jinyun Song |
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| Institution: | 1. Jiangsu Key Laboratory for Molecular and Medical Biotechnology, Life Science College, Nanjing Normal University, Nanjing, P.R. China;2. Basic Medical College, Nanjing University of Chinese Medicine, Nanjing, P.R. Chinashuangquanz@yahoo.com;4. wuht@njutcm.edu.cn;5. College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, P.R. China |
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| Abstract: | A proliferation inducing ligand (APRIL) is a member of the TNF superfamily. It shares two receptors with B-cell activating factor (BAFF), B-cell maturation antigen (BCMA), and transmembrane activator and CAML interactor (TACI). Herein, the equine APRIL was identified from equine adipose-derived stem cell (ASC), and the protein expression of APRIL and its related molecules were detected during the adipogenic differentiation of equine ASC in vitro. The equine APRIL gene was located on chromosome 11, spans 1852 base pairs (bp). Its open reading frame covers 753 bp, encoding a 250-amino acid protein with the typical TNF structure domain. During the two weeks’ adipogenic differentiation of equine ASC, although the protein expression of APRIL and TACI had an insignificant change, that of BCMA increased significantly. Moreover, with the addition of recombinant protein His6-sAPRIL, a reduced differentiation of equine ASC toward adipocyte was detected. These results may provide the basis for investigating the role of APRIL in ASC adipogenic differentiation. |
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| Keywords: | Adipogenic differentiation adipose-derived stem cell APRIL equine |
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