Intracellular Ca2+ imaging for micropatterned cardiac myocytes |
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Authors: | Kaji Hirokazu Takoh Kimiyasu Nishizawa Matsuhiko Matsue Tomokazu |
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Institution: | Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Sendai 980-8579, Japan. |
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Abstract: | The patterning of cardiac myocytes on a micron scale ( approximately 5 microm) was achieved by microcontact printing of fibronectin onto a hydrophobically pretreated glass substrate. The patterned cardiac myocytes conjugated with each other by forming a gap junction, as judged from the synchronized Ca(2+) transition over the pattern, and thus simultaneously contracted. The dynamic change of the Ca(2+) concentration within the patterned tissue was analyzed quantitatively during successive contraction and relaxation using a Nipkow-type high-speed confocal microscope. |
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Keywords: | cardiac myocytes microcontact printing (μCP) pattering confocal microscope Ca2+ imaging |
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