Human pepsinogen A (PGA): an informative gene complex located at 11q13 |
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Authors: | Farzin H. Boudi Ragnhild A. Lothe R. Thomas Taggart |
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Affiliation: | (1) Department of Molecular Biology and Genetics, Wayne State University School of Medicine, 540 East Canfield, 48201 Detroit, MI, USA;(2) Molecular Biology Center, Wayne State University, 48201 Detroit, MI, USA;(3) Det Norske Radiumhospitalet, Montebello, N-0310 Oslo, Norway;(4) Department of Molecular Biology and Genetics, 3216 Scott Hall, Wayne State University School of Medicine, 540 East Canfield, 48201 Detroit, MI, USA |
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Abstract: | Summary Human pepsinogen (PGA) exhibits extensive polymorphism that can be detected both at the protein and the DNA level. We describe here two restriction fragment length polymorphisms, EcoRI and BglII, which provide for the detection of three of the most common PGA haplotypes (A, B, and C) in the United States population. The relationship of these polymorphisms to each PGA haplotype was determined by analysis of DNA from individuals exhibiting the corresponding protein phenotypes and by analysis of a series of human × mouse somatic cell hybrids containing the individual chromosome 11 homologous from heterozygous individuals exhibiting the AB and AC protein phenotypes. The use of the BglII polymorphism in combination with previously described EcoRI polymorphism provides a very informative marker of 11q13. |
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