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The transport of an intact oligopeptide across adult mammalian jejunum
Authors:K Takaori  J Burton  M Donowitz
Institution:1. Laboratory of Cellular and Molecular Research, Massachusetts General Hospital, Boston, MA 02114 USA;2. Biomolecular Medicine, University Hospital, Boston, MA 02118 USA;3. Department of Medicine, New England Medical Center, Boston, MA 02111 USA;4. Department of Physiology, New England Medical Center, Boston, MA 02111 USA;5. Tufts University School of Medicine, Boston, MA 02111 USA;1. APC Microbiome Ireland, University College Cork, Cork, Ireland;2. School of Pharmacy, University College Cork, Cork, Ireland;3. School of Microbiology, University College Cork, Cork, Ireland;4. School of Biochemistry and Cell Biology, University College Cork, Cork, Ireland;1. Respiratory Technology, Woolcock Institute of Medical Research and Discipline of Pharmacology, Sydney Medical School, Sydney, Australia;2. Imperial College Healthcare NHS Trust, London, UK;3. Department of Life Sciences and Biotechnology, University of Ferrara, Via Fossato di Mortara 17/19, 44121 Ferrara, Italy;1. Department of Genetic Medicine and Development, Faculty of Medicine, University of Geneva, 1211 Geneva, Switzerland;2. iGE3, Institute of Genetics and Genomics of Geneva, University of Geneva, 1211 Geneva, Switzerland;3. SIB, Swiss Institute of Bioinformatics, University of Geneva, 1211 Geneva, Switzerland;1. Graduate Institute of Pharmaceutical Sciences, School of Pharmacy, National Taiwan University, Taipei, Taiwan;2. Drug Research Center, College of Medicine, National Taiwan University, Taipei, Taiwan;3. Institute of Chemistry, Academia Sinica, Nankang, Taipei, Taiwan;1. Department of Biology, Dalhousie University, Halifax, NS B3H4R2, Canada;2. Department of Biology, University of Washington, Seattle, WA 98195, USA
Abstract:The passage of an intact nonapeptide across adult rabbit jejunum mounted in an Ussing Chamber is demonstrated with an HPLC system which resolves the renin inhibitor Pro-His-Pro-Phe-His-Leu-Phe-Val-3H]Phe from all labelled proteolytic cleavage products. Permeability of the peptide (0.016 cm hr-1) is approximately one-seventh that observed for the actively transported 3-0-methyl glucose (0.104 cm hr-1). Flux of the peptide is not changed by the absence of sodium. This study shows that adult mammalian intestine can transport intact oligopeptides and suggests that it will be feasible to develop orally active peptide drugs.
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