H9N2型禽流感病毒传播途径分子机制的研究

选择禽流感病毒(avian influenza virus,AIV)A/chicken/Zhuhai/154/2003(H9N2)(AZHl54)株作为骨架病毒,与来自A/chicken/Guangdong/SS/94(H9N2)(SS94)AIV的NA基因和HA基因进行H9N2亚型重排.动物传播性实验发现:AZH154、SS94和3株重排的H9N2亚型AIV都可以经直接接触途径传播;5株H9N2 AIV都不经过粪便接触传播;且AZH154株和重组的H9N2亚型AZH154/SSHA能经过气溶胶传播.实验结果表明:AIV(H9N2)的NA基因与该病毒气溶胶传播途径有重要关系,即2003年珠海地区AIV(H9N2)的大流行可能是因为病毒获得气溶胶传播途径的特性,且病毒的NA基因发挥了重要的作用.

Research of Molecular Mechanism Affecting Transmission Ways for H9 N2 AIV

H9N2 subtype influenza virus A/chicken/Zhuhai/154/2003(AZH154)strain was chosen as the master strain for research.In order to investigate the molecular mechanism affecting transmission ways for H9N2 AIVs,AZH154 and A/chicken/Guangdong/SS/94(H9N2)(SS94)were compared in their transmission ways.The findings suggested that AZH154 strain could be transmitted in chickens by direct contact and by aerosol route.Whereas SS94 strain only by direct contact,and neither of two viruses by fecal contact.To determine the genes associated with the ability to transmit by aerosol route in chickens,a set of transmission experiments were designed.Groups of three chickens were inoculated with equal dose of virus by oral,intracheal and intranasal routes.Virus isolation and identification showed that only the AZH154/SSHA recombinants were transmitted from inoculated to uninoculated chickens by aerosol route,whereas three recombinants were transmitted by direct contact,but not by fecal contact.The data suggest that the NA gene might be the major determinant of the ability of aerosol transmission for H9N2 subtype AIVs in Zhuhai in 2003.