Experimental Study on the Suppression of Human Nuclear Receptor hLRH-1 via a Vector-based RNA Interference |
| |
Authors: | Shui-Liang WANG Ji-Liang FU |
| |
Institution: | a PLA Center for Laboratory Medicine, Fuzhou General Hospital, Fuzhou 350025, China b Department of Medicine and Life Science, Tongji University, Shanghai 200092, China |
| |
Abstract: | To explore the inhibitions of human nuclear receptor hLRH-1 via RNA interference, siRNAs expressing vectors pShLRH-1.1 and pShLRH-1.2, and targeting hLRH-1 were designed and constructed. The recombinants were introduced into hepatocellular carcinoma cells, BEL-7402, mediated by lipofectaminTM. RT-PCR was carried out to examine the inhibition ratio of hLRH-1 expression. The same method was also applied to analyze the expression of farnesyl pyrophosphate synthetase (FPPS) gene. Our results demonstrated that after transient transfection, both pShLRH-1.1 and pShLRH-1.2 could trigger the efficient inhibition of hLRH-1 in cultured cells, BEL-7402. The inhibition ratios were up to 80%. By comparing with non-transfection and vector-transfection control, the expression of FPPS in cells with inhibition of hLRH-1 was up-regulated significantly. Thus, the inhibition of expression of hLRH-1 in cultured cells was achieved via RNA interference in this study. Our results also suggested that hLRH-1 acts as a negative regulator in FPPS expression. |
| |
Keywords: | RNA interference nuclear receptor hLRH-1 suppression of gene expression |
本文献已被 ScienceDirect 等数据库收录! |
|