Disclosure by triton X-100 of NMDA-sensitive [3H] glutamate binding sites in brain synaptic membranes |
| |
Authors: | K Ogita Y Yoneda |
| |
Institution: | Department of Pharmacology, Setsunan University, Osaka, Japan. |
| |
Abstract: | Pretreatment of brain synaptic membrane homogenates with Triton X-100 resulted in a drastic disclosure of 3H] glutamate (Glu) binding activity which was sensitive to one of the central Glu receptor agonists, N-methyl-D-aspartic acid (NMDA). The NMDA-sensitive binding was inversely dependent on the incubation temperature, and was a reversible and saturable process. Scatchard analysis revealed that Triton X-100 treatment yielded in a significant enhancement of the affinity with a concomitant increment of the density of binding sites. Electrophysiologically identified agonists and antagonists for the NMDA receptors all significantly inhibited the binding to Triton-treated membranes. These results suggest that Triton-treatment may disclose NMDA-sensitive 3H] Glu binding sites in brain synaptic membranes. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|