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Identification of Cytokinins in Root Exudate of the Rice Plant
Authors:Murofushi, Noboru   Inoue, Ayumu   Watanabe, Naoharu   Ota, Yasuo   Takahashi, Nobutaka
Affiliation:1Department of Agricultural Chemistry, The University of Tokyo Bunkyo-ku, Tokyo 113, Japan
2National Institute of Agricultural Sciences Yatabe, Ibaraki 305, Japan
Abstract:Cytokinins, cis-zeatin and cis- and (trans-ribosylzeatin, wereidentified in the root exudate of the rice plant (Oryza sativa,indica cultivar IR-24) after several chromatographic separationsand combined gas-liquid chromatography-selected ion monitoring(GC-SIM) analysis. The presence of trans-zeatin ribotide wassuggested by enzyme hydrolysis, subsequent chromatographic separationand GC-SIM. The comparatively high content of the ribotide inthe root exudate suggests the form of cytokinins to be transportedfrom roots to other parts in the rice plant. (Received July 22, 1982; Accepted November 25, 1982)
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