Characterization of alpha-1-antitrypsin by isoelectric focusing on an ultrathin polyacrylamide gel layer |
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| Authors: | G Massi A Fabiano D Ragusa P Auconi |
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| Institution: | (1) Istituto di Anatomia e Istologia Patologica, Università Cattolica del Sacro Cuore, via Pineta Sacchetti 644, I-00168 Roma, Italy;(2) Divisione di Pediatria, Ente Ospedaliero di Arezzo, Arezzo, Italy |
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| Abstract: | Summary Through the use of ultrathin layer polyacrylamide gel isoelectric focusing it is possible to obtain a resolution of the bands of  1AT so as to be able to easily recognize all six PiM subtypes. The optimal resolution of the PiM subtypes is obtained without deforming the pattern of the Pi phenotypes. In addition to high resolution, ultrathin layer polyacrylamide gel isoelectric focusing permits a notable reduction of fees to 1/5 of the usual. |
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