|
|||||
|
|
Fmoc-based solid phase chemical synthesis of 71-meric neuregulin 1-beta1, an epidermal growth factor-like domain. |
|
| Authors: | Taeko Kakizawa Shizuyo Koide-Yoshida Tooru Kimura Hiromasa Uchimura Yoshio Hayashi Kazuki Saito Yoshiaki Kiso |
| Institution: | Department of Medicinal Chemistry, Center for Frontier Research in Medicinal Science, 21st Century COE Program, Kyoto Pharmaceutical University, Yamashina-ku, Kyoto 607-8412, Japan. |
| Abstract: | The human neuregulin 1-beta1 (NRG1-beta1, amino acid residues 176-246) was chemically synthesized by Fmoc-based solid phase peptide synthesis (SPPS) followed by folding in a redox buffer. The biological activity of the synthesized NRG1-beta1 was confirmed by ligand-induced tyrosine phosphorylation on Chinese hamster ovary (CHO) cells expressing ErbB-4. |
| Keywords: | neuregulin 1‐β1 solid phase peptide synthesis fluorenylmethoxycarbonyl group ErbB‐4 |