Development of ten new EST-derived microsatellites in Atlantic cod (Gadus morhua L.) |
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Authors: | Jon-Ivar Westgaard Tekle Tafese Mette Serine Wesmajervi Frank Nilsen Kjersti Turid Fjalestad Børge Damsgård Madjid Delghandi |
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Affiliation: | 1. Norwegian College of Fishery Science, Troms?, Norway 2. Department of Aquaculture, Norwegian Institute of Fisheries and Aquaculture Research, Muninbakken 9-13, Breivika, PO box 6122, Troms?, 9291, Norway 3. Institute of Marine Research, Bergen, Norway
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Abstract: | Ten polymorphic microsatellite markers were developed from approximately 1,300 expressed sequence tags (ESTs) of Atlantic cod (Gadus morhua L.). Thirty two primer pairs were designed for EST sequences containing perfect di- tri- tetra- and pentanucleotide motifs and characterised in 96 unrelated fish. Ten markers were successfully amplified with number of alleles from 2 to 13 per locus and observed and expected heterozygosity ranging from 0.03 to 0.69 and 0.03 to 0.74, respectively. Loci Gmo-C131, C132 and C136 deviated from Hardy-Weinberg equilibrium. Genetic linkage disequilibrium analysis between all pairs of the loci showed significant departure from the null hypothesis between loci Gmo-C131 and Gmo-C132 and C128 and Gmo-C133. The gene identity was determined at five of the loci, confirming the associated microsatellites as Type I markers. The new microsatellites reported in this work can be used for conservation and enhancement of wild stocks for commercial harvesting. Jon-Ivar Westgaard and Tekle Tafese have contributed equally to the work. |
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Keywords: | Atlantic cod Expressed sequence tag Microsatellite Type I marker |
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