Characterization of a polyclonal anti-peptide antibody to the angiotensin II type-1 (AT1) receptor. |
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| Authors: | B Zelezna E M Richards W Tang D Lu C Sumners M K Raizada |
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| Institution: | Department of Physiology, College of Medicine, University of Florida, Gainesville 32610. |
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| Abstract: | A polyclonal antibody has been prepared against a synthetic peptide corresponding to amino acids 14-23 of the angiotensin II type-1 (AT1) receptor. The antibody is of high titer and mono-specific. Western blot analysis of membranes from rat liver, kidney, and adrenal gland showed that the antibody specifically recognizes a protein band of MW 70,000 whose amounts are highest in the liver, followed by kidney and adrenals. In addition, a relatively less prominent band of MW 95,000 was also detected. The relative distribution of this protein correlates well with the values obtained for 3H]-DuP753 binding and AT1 receptor mRNA. |
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