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High forskolin production in hairy roots of Coleus forskohlii |
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| Authors: | K. Sasaki A. Udagawa H. Ishimaru T. Hayashi A. W. Alfermann F. Nakanishi K. Shimomura |
| Affiliation: | Department of Bioscience and Biotechnology, Faculty of Engineering, Aomori University, 2-3-1, Kohbata, Aomori 030, Japan Fax no.: +81-177-38-2030 E-mail: ksasaki@aomori-u.ac.jp, JP Biological Science Research Center, Lion Corporation, 100 Tajima, Odawara, Kanagawa 256, Japan, JP Heinrich-Heine-Universit?t Düsseldorf, Institut für Entwicklungs- und Molekularbiologie der Pflanzen, Universit?tsstra?e 1, Geb. 26.13, D-40225 Düsseldorf 1, Germany, DE Tsukuba Medicinal Plant Research Station, National Institute of Health Sciences, 1 Hachimandai, Tsukuba, Ibaraki 305, Japan, JP |
| Abstract: | Hairy roots of Coleus forskohlii were induced by infection with the Agrobacterium rhizogenes MAFF 03-01724 strain. Growth and forskolin production of two hairy root clones cultured in various liquid media were examined. Hairy root clone B9 grew well in woody plant liquid medium and showed a high forskolin yield (ca. 1.3 mg/ 100 ml flask) after 5 weeks of culture. The time course of growth and forskolin production of the clone B9 cultured in woody plant liquid medium was also examined. Rapid growth started at week 2 and continued until week 5. The highest forskolin yield (ca. 1.6 mg/100 ml flask) was obtained at week 5. Productivity was much higher than that previously reported. Received: 19 June 1997 / Revision received: 6 October 1997 / Accepted: 18 October 1997 |
| Keywords: | Forskolin Coleus forskohlii Hairy root |
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