Growth and macromolecular content of the dimorphic fungus Aureobasidium pullulans and the effect of hydroxyurea and other inhibitors |
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Authors: | Maria J. Sevilla M. Dolores Moragues Federico Uruburu |
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Affiliation: | (1) Departamento de Biología, Facultad de Ciencias, Universidad del País Vasco, Apartado 644, 48080 Bilbao, (Spain);(2) Departamento de Microbiología, Facultad de Ciencias Biológicas, Universidad de Valencia, Burjasot, 46100 Valencia, (Spain) |
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Abstract: | The growth kinetics and the macromolecular content of the yeast and ethanol-induced hyphal forms of Aureobasidium pullulans were studied. During the morphological transition from yeasts to hyphae, both the protein and RNA content decreased significantly, the mycelial form containing only 76% of the amount of protein in the yeasts, and 38% of the RNA. The DNA was the only component tested whose level increased during the transition. Among several compounds inhibiting macromolecular synthesis, only hydroxyurea showed a remarkable effect on the morphology of A. pullulans, inducing the mycelial morphology. The macromolecular composition of hydroxyurea-treated cultures changed with time in a way similar to that of the ethanol-Tween 80-ammonia medium, and to that of carbon-starved cultures, without ethanol or glucose. |
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Keywords: | Aureobasidium pullulans Pullularia pullulans dimorphism growth macromolecules hydroxyurea |
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