Proteinic prorenin-releasing-stimulator (PRS) in the rat submandibular gland |
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| Authors: | F Suzuki S Miyazaki M Uozumi N Okamoto S Yamashita K Murakami Y Nakamura |
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| Affiliation: | Department of Biotechnology, Faculty of Agriculture, Gifu University, Japan. |
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| Abstract: | The release of prorenin as well as renin from rat renal slices was confirmed by a rat prorenin-prosegment ELISA system and an assay system for determining the renin activity. A significant increase of the prorenin release was found by adding rat submandibular gland extract to the slice medium, indicating the existence of a prorenin-releasing stimulator (PRS) in the extract. The pI and molecular mass of PRS were 8.5-8.7 and 28-30 kDa, respectively. The PRS was completely inactivated by boiling or a proteinase treatment. |
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