基因打靶方法制备乳腺生物反应器

基因打靶技术制备乳腺生物反应器,克服了显微注射法的众多缺陷,并随着第一例基因打靶家畜的诞生而成为这一研究领域的热点。本文从制备乳腺生物反应器过程中基因打靶策略、打靶细胞到打靶位点的选择等各个方面,详细分析了其中的技术难点、解决问题的对策、国内外研究进展以及应用前景。

Producing Mammary Gland Bioreactor Using Gene Targeting

Producing mammary gland bioreactor using gene targeting shows great advantage over pronuclear micro-injection, and is becoming a research hotspot in this area after the first gene targeted sheep was born. The technical challenges, the solution, current developments and the prospects for the selection of gene targeting strategies, targeting cells and targeting gene locus in producing mammary gland bioreactor were discussed in details. ;