The rpoD gene functions as a multicopy suppressor for mutations in the chaperones, CbpA, DnaJ and DnaK, in Escherichia coli |
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Authors: | Tadasu Shiozawa Chiharu Ueguchi Takeshi Mizuno |
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Affiliation: | Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University, Chikusa-ku, Nagoya 464, Japan |
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Abstract: | Abstract The CbpA protein is an analog of the DnaJ molecular chaperone of Escherichia coli . The dnaJ − cbpA − double-null mutant exhibits severe defects in cell growth, namely, a very narrow temperature range for growth. To gain insight into the functions of CbpA as well as DnaJ, we isolated a multicopy suppressor gene that permits this dnaJ − cbpA −~ mutant to grow normally at low temperatures. The suppressor gene was identified as rpoD , the gene that encodes the major σ 70. The biological implications of this finding are examined and discussed. |
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Keywords: | Heat shock response Molecular chaperone CbpA of E. coli DnaK and DnaJ chaperones Sigma subunit |
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