Functional characterization of <Emphasis Type="Italic">Vibrio cholerae</Emphasis> O1 WbeW enzyme responsible for initial reaction in O antigen biosynthesis |
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Authors: | Chang Sup Kim Jeong Hyun Seo Hyung Joon Cha |
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Institution: | 1.School of Chemistry and Biochemistry,Yeungnam University,Gyeongsan,Korea;2.School of Chemical Engineering,Yeungnam University,Gyeongsan,Korea;3.Department of Chemical Engineering,Pohang University of Science and Technology,Pohang,Korea |
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Abstract: | Vibrio cholerae O1 employs the ATP-binding cassette (ABC) transporter-dependent pathway for O antigen biosynthesis. Different from highly studied Klebsiella pneumoniae and Escherichia coli, it was reported that initial reaction of O antigen biosynthesis in V. cholerae O1 may be involved in WbeW protein, which is predicted to be a galactosyltransferase. In this work, we report expression and characterization of WbeW enzyme. WbeW was expressed as membrane-associated form in E. coli and it was obtained with high purity. The enzyme had a function of transferring Gal-1-P from UDP-Gal to Und-P, implying that initial glycan of O antigen in V. cholerae O1 can be composed of a Gal residue. |
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