Application of an M13 bacteriophage displaying tyrosine on the surface for detection of Fe3+ and Fe2+ ions |
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Authors: | Xiaohua Guo Chuncheng Niu Yunhua Wu Xiaosheng Liang |
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Institution: | 1. College of Life Science & Hubei Provincial Key Laboratory for Protection and Application of Special Plants on Wuling Area, South-Central University for Nationalities, Wuhan, 430074, China
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Abstract: | Ferric and ferrous ion plays critical roles in bioprocesses, their influences in many fields have not been fully explored due to the lack of methods for quantification of ferric and ferrous ions in biological system or complex matrix. In this study, an M13 bacteriophage (phage) was engineered for use as a sensor for ferric and ferrous ions via the display of a tyrosine residue on the P8 coat protein. The interaction between the specific phenol group of tyrosine and Fe3+ / Fe2+ was used as the sensor. Transmission electron microscopy showed aggregation of the tyrosine-displaying phages after incubation with Fe3+ and Fe2+. The aggregated phages infected the host bacterium inefficiently. This phenomenon could be utilized for detection of ferric and ferrous ions. For ferric ions, a calibration curve ranging from 200 nmol/L to 8 μmol/L with a detection limit of 58 nmol/L was acquired. For ferrous ions, a calibration curve ranging from 800 nmol/L to 8 μmol/L with a detection limit of 641.7 nmol/L was acquired. The assay was specific for Fe3+ and Fe2+ when tested against Ni2+, Pb2+, Zn2+, Mn2+, Co2+, Ca2+, Cu2+, Cr3+, Ba2+, and K+. The tyrosine displaying phage to Fe3+ and Fe2+ interaction would have plenty of room in application to biomaterials and bionanotechnology. |
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Keywords: | M13 bacteriophage tyrosine display ferric ion ferrous ion aggregation |
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