Thermostable lipolytic enzymes production in batch and continuous cultures of Thermus thermophilus HB27 |
| |
Authors: | Alberto Domínguez Francisco J Deive Lorenzo Pastrana Maria L Rúa Maria A Longo M Angeles Sanroman |
| |
Institution: | (1) Department of Chemical Engineering, University of Vigo, Vigo, Spain;(2) Department of Food and Analytical Chemistry, University of Vigo, Vigo, Spain; |
| |
Abstract: | Several studies in laboratory-scale bioreactors are undertaken in order to verify the beneficial effect of thermal spring
water in the culture medium of Thermus thermophilus HB27. Two bioreactor configurations, stirred tank and airlift, are investigated to determine the most suitable one to develop
a continuous process. Water mineral composition affects the lipolytic enzyme secretion and growth of T. thermophilus HB27 in both bioreactor configurations. Furthermore, the lipolytic activity is strongly enhanced when stirred tank bioreactor
is used. Moreover, operation in a stirred tank at an agitation rate of 650 rpm leads to the highest total lipolytic activity
(intra- and extracellular enzyme) around 280 U/L after 32 h. Continuous cultures operating in the optimised conditions determined
in batch cultures are carried out. It is noticeable that the stirred tank bioreactor was able to operate in a continuous flow
mode without operational problems. In addition, the lipolytic activity obtained is about 2-fold higher than that attained
in batch cultures. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|